A wash sale occurs when you acquire “substantially identical” stocks or securities within 30 days before or 30 days after realizing a loss. The key words here are "replacement securities". The IRS has no issue if you sell for a gain and buy back identical stocks or securities within the above time frame. The purpose of the wash sale rule is to prevent investors/traders from creating artificial losses in order to reduce taxation. For example:
1) 2/02/20 buy 100 shares @ $20
2) 3/10/20 buy 100 shares @ $14
3) 3/11/20 sell 100 shares @ $15
If FIFO is used, the first and third trades result in a $5 loss. The second trade creates the wash sale because they are considered replacement shares. Therefore the $5 loss must be deferred until the second purchase is sold.
In your example, there are is no wash sale because all shares have been disposed of. If your second purchase was within 30 days of the 3/01/20 sale date, there would still be no wash sale because no replacement shares were purchased (you have no ongoing open position).